Singleron Custom FocuSCOPE Single Cell Multiomics Kit, 16RXNs, EA, 3000012-2

The Singleron FocuSCOPE Single Cell Multiomics Kit is a targeted single-cell sequencing solution for simultaneous whole-transcriptome profiling and targeted detection of sequence variants, gene fusions, and viral sequences from the same single cells. The kit uses barcoding beads with both poly(dT) and customizable target probes to generate two libraries (whole transcriptome and enrichment) from the same cell population and supports streamlined sample-to-library processing within one workday. Primary use cases include cancer research, viral studies, and investigation of disease mechanisms at single-cell resolution.

Key Features:

• Unit size: 16 RXNs
• Manufacturer part code: 3000012-2
• Format: EA (each)
• Captures up to 30,000 cells per run (chip-dependent; SD or HD chip selectable)
• Up to 10 customizable target regions (pre-validated panels available; target regions up to ~200 bp)
• Two libraries generated per experiment: whole transcriptome library + targeted enrichment library
• Recommended sequencing: Paired-End 150; sequencing depth 20,000–50,000 read pairs/cell for transcriptome and ~5,000 reads/cell for enrichment

Advantages:

• Simultaneous detection of transcriptome and sequence variants (SNVs, fusions, viral sequences) from the same single cells for integrated genotype–phenotype analysis
• Customizable target probes and ready-to-use pre-validated panels to focus on genes or regions of interest
• Streamlined, instruments-compatible workflow that produces two libraries in a single day, enabling faster experimental turnaround
• High sensitivity for hotspot mutation detection and capability to enrich low-abundance target sequences while preserving whole-transcriptome data